Next-generation sequencing data analysis / Xinkun Wang.

Includes bibliographical references and index.

Section 1. Introduction to cellular and molecular biology --
section 2. Introduction to next-generation sequencing (NGS) and NGS data analysis --
section 3. Application-specific NGS data analysis --
section 4. The changing landscape of next-generation sequencing technologies and data analysis.

The book walks readers through the multiple stages of NGS data generation and analysis in an easy-to-follow fashion. It covers every step in each stage, from the planning stage of experimental design, sample processing, sequencing strategy formulation, the early stage of base calling, reads quality check and data preprocessing to the intermediate stage of mapping reads to a reference genome and normalization to more advanced stages specific to each application.